Students learn to calibrate a microscope, calculate magnification, and see how the light path affects the proper use of both the field and aperture diaphragms.
Combining theory and hands-on exercises allows students to properly set up Koehler illumination, understand how interpupillary distance and diopter settings impact images, and see how to maintain and clean an instrument.
- Components of the microscope
- Setting up Köehler illumination
- How to use the microscope
- Illumination techniques and types of filters
- Calibration and micrometry
- Basic care and cleaning of the microscope
Upon satisfactory completion of this course the student will be able to:
- Identify the major components of the microscope and describe the function of each
- Discuss the role of Köehler illumination in microscopy and adjust a microscope for Köehler-like illumination
- Properly set up the microscope for use
- Describe how to correctly focus the microscope to obtain the best image
- Identify and set up appropriate illumination
- Use an ocular micrometer to measure specimens viewed with the microscope
- Troubleshoot problems commonly encountered when using a microscope
All instrumentation, materials, and supplies necessary for successful completion of this course will be provided onsite by Hooke College of Applied Sciences.
- Individuals who have little or no experience with properly using an advanced industrial or research grade microscope
- Users who recognize that they and their work, will benefit from increasing their knowledge and proficiency with a microscope
- Teachers and students who want to enhance their teaching/learning by getting the most form the tools and instruments they use
Students are expected to successfully complete a variety of tasks in the form of hands-on exercises, laboratory exercises, identifications of unknowns, and quizzes. Students are notified at the end of the course whether or not they have successfully completed the requirements of the course based on:
- 100% Attendance
- Class participation
- Completion of all course material
- Completed and signed student evaluation form
Upon successfully meeting these requirements, a student is awarded a certificate of completion and CEU credits, if available. Those who have not successfully passed the course requirements do not receive a certificate or CEU credits.