Basic Microscopy

Course Details

Students learn to calibrate a microscope, calculate magnification, and see how the light path affects the proper use of both the field and aperture diaphragms.

Combining theory and hands-on exercises allows students to properly set up Koehler illumination, understand how interpupillary distance and diopter settings impact images, and see how to maintain and clean an instrument.

  • Components of the microscope
  • Setting up Köehler illumination
  • How to use the microscope
  • Illumination techniques and types of filters
  • Calibration and micrometry
  • Basic care and cleaning of the microscope

Upon satisfactory completion of this course the student will be able to:

  • Identify the major components of the microscope and describe the function of each
  • Discuss the role of Köehler illumination in microscopy and adjust a microscope for Köehler-like illumination
  • Properly set up the microscope for use
  • Describe how to correctly focus the microscope to obtain the best image
  • Identify and set up appropriate illumination
  • Use an ocular micrometer to measure specimens viewed with the microscope
  • Troubleshoot problems commonly encountered when using a microscope

All instrumentation, materials, and supplies necessary for successful completion of this course will be provided onsite by Hooke College of Applied Sciences.

  • Individuals who have little or no experience with properly using an advanced industrial or research grade microscope
  • Users who recognize that they and their work, will benefit from increasing their knowledge and proficiency with a microscope
  • Teachers and students who want to enhance their teaching/learning by getting the most form the tools and instruments they use

No prerequisites.

Students are expected to successfully complete a variety of tasks in the form of hands-on exercises, laboratory exercises, identifications of unknowns, and quizzes. Students are notified at the end of the course whether or not they have successfully completed the requirements of the course based on:

  • 100% Attendance
  • Class participation
  • Completion of all course material
  • Completed and signed student evaluation form

Upon successfully meeting these requirements, a student is awarded a certificate of completion and CEU credits, if available. Those who have not successfully passed the course requirements do not receive a certificate or CEU credits.

Please see our General Policies page for information related to course registration and cancellation.


Accredited as an Authorized Provider by the International Association for Continuing Education and Training (IACET), an internationally recognized organization for standards and authorization for continuing education and training, Hooke College of Applied Sciences provides a research-based learning experience taught by some of the best experts in the industry.

Speak to an Admissions Representative

Our representatives are here to answer any questions you have about a course or program at Hooke College.