Sample Preparation Techniques

Course Details

This course focuses on isolating and manipulating 1-100 µm sized particles without the use of micromanipulators. Sample preparation techniques for isolating contaminants from liquids, solids and surfaces are covered. Students spend more than 85% of class time practicing sample preparation under the supervision of the instructor. Some of the sample handling supplies made in class are retained by the students for their own use.

Methods demonstrated and discussed include sample preparation for:

This course is part of our Industrial Microscopy Specialization.

  • Introduction and overview of characterizing and preparing micrometer size particles
  • Instruction and practice in microanalytical tool fabrication
  • Instruction and practice in using fabricated microanalytical tools
  • Methods for isolation of contamination from liquids, solids and surfaces
  • Instruction and practice in preparing samples for polarized light microscopy, FTIR/Raman, and EDS/SEM
  • Upon completion of this course, the student will be able to successfully isolate and manipulate particles sized 1-100 µm from liquids, solids, and surfaces for future analysis by polarized light microscopy, FTIR/Raman, and EDS/SEM
  • The student will be able to operate and use a stereomicroscope and microanalytical tools effectively in sample preparation
  • Instructed in microanalytical tool fabrication, the student will learn how to produce tools correctly to aid in the isolation, mounting, and handling of micro particles
  • Equipped with instruments, microanalytical tools, and techniques, the student will successfully isolate particles from liquids, semi-soft solids and surfaces
  • Provided with techniques helpful to the operation and use of microscopical instrumentation and microanalytical tools, the student will perform precise isolation and manipulation of micrometer size particles
  • This course is directed towards students who would like to learn the precise methods of particle isolation and manipulation
  • Professionals that must prepare, isolate, or manipulate particles for a wide range of analysis techniques will benefit from attending this class

All instrumentation, materials, and supplies necessary for successful completion of this course will be provided onsite by Hooke College of Applied Sciences.

  • Detailed course manual
  • Olympus SZX10 stereomicroscope for each student
  • Olympus BX51 polarized light microscope
  • Undergraduate science background and analytical chemistry
  • Students should be familiar with polarized light microscopy and stereomicroscopy, and micrometry as applied to microscopic specimens

Students are expected to successfully complete a variety of tasks in the form of hands-on exercises, laboratory exercises, identifications of unknowns, and quizzes. Students are notified at the end of the course whether or not they have successfully completed the requirements of the course based on:

  • 100% Attendance
  • Class participation
  • Completion of all course material
  • Completed and signed student evaluation form

Upon successfully meeting these requirements, a student is awarded a certificate of completion and CEU credits, if available. Those who have not successfully passed the course requirements do not receive a certificate or CEU credits.

Please see our General Policies page for information related to course registration and cancellation.

This course is part of the Industrial Microscopy Specialization program.

The program consists of attending four standard Hooke College of Applied Sciences courses with hands-on instruction and a 40 hour practicum study characterizing unknown samples.

  1. Polarized Light Microscopy
  2. Scanning Electron Microscopy
  3. Sample Preparation Techniques
  4. Infrared Microscopy

After finishing the four courses, students complete a Capstone Project that requires students to characterize and identify samples by integrating all of the skills learned during the four courses taken for the program. Those students who successfully complete the four courses and associated practicums and a Capstone Project earn an Industrial Microscopy Specialization.

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Accredited as an Authorized Provider by the International Association for Continuing Education and Training (IACET), Hooke College of Applied Sciences provides a research-based, hands-on learning experience taught by some of the best experts in the industry. From continuing education to custom courses and professional development, Hooke College will allow you to gain the skills you need.