This course focuses on isolating and manipulating 1-100 µm sized particles without the use of micromanipulators. Sample preparation techniques for isolating contaminants from liquids, solids and surfaces are covered. Students spend more than 85% of class time practicing sample preparation under the supervision of the instructor. Some of the sample handling supplies made in class are retained by the students for their own use.
Methods demonstrated and discussed include sample preparation for:
This course is part of our Industrial Microscopy Specialization.
- Introduction and overview of characterizing and preparing micrometer size particles
- Instruction and practice in microanalytical tool fabrication
- Instruction and practice in using fabricated microanalytical tools
- Methods for isolation of contamination from liquids, solids and surfaces
- Instruction and practice in preparing samples for polarized light microscopy, FTIR/Raman, and EDS/SEM
- Upon completion of this course, the student will be able to successfully isolate and manipulate particles sized 1-100 µm from liquids, solids, and surfaces for future analysis by polarized light microscopy, FTIR/Raman, and EDS/SEM
- The student will be able to operate and use a stereomicroscope and microanalytical tools effectively in sample preparation
- Instructed in microanalytical tool fabrication, the student will learn how to produce tools correctly to aid in the isolation, mounting, and handling of micro particles
- Equipped with instruments, microanalytical tools, and techniques, the student will successfully isolate particles from liquids, semi-soft solids and surfaces
- Provided with techniques helpful to the operation and use of microscopical instrumentation and microanalytical tools, the student will perform precise isolation and manipulation of micrometer size particles
- This course is directed towards students who would like to learn the precise methods of particle isolation and manipulation
- Professionals that must prepare, isolate, or manipulate particles for a wide range of analysis techniques will benefit from attending this class
All instrumentation, materials, and supplies necessary for successful completion of this course will be provided onsite by Hooke College of Applied Sciences.
- Detailed course manual
- Olympus SZX10 stereomicroscope for each student
- Olympus BX51 polarized light microscope
- Undergraduate science background and analytical chemistry
- Students should be familiar with polarized light microscopy and stereomicroscopy, and micrometry as applied to microscopic specimens
Students are expected to successfully complete a variety of tasks in the form of hands-on exercises, laboratory exercises, identifications of unknowns, and quizzes. Students are notified at the end of the course whether or not they have successfully completed the requirements of the course based on:
- 100% Attendance
- Class participation
- Completion of all course material
- Completed and signed student evaluation form
Upon successfully meeting these requirements, a student is awarded a certificate of completion and CEU credits, if available. Those who have not successfully passed the course requirements do not receive a certificate or CEU credits.