Sample Preparation Techniques

Course Details


This course focuses on isolating, mounting, and handling 1-100 µm sized particles without the use of micromanipulators. Sample preparation techniques for isolating contaminants from liquids, solids, or surfaces are covered. Students spend more than 50% of class time practicing sample preparation under the supervision of the instructor. Some of the sample-handling supplies made in class are retained by the students for their own use.

Methods demonstrated and discussed include sample preparation for:

This course is part of our Industrial Microscopy Specialization.

  • Introduction and overview of characterizing and preparing micrometer particles
  • Instruction and practice in microtool fabrication
  • Instruction and practice in using fabricated micro tools
  • Instruction and practice in preparing samples for polarized light microscopy, FTIR, and SEM
  • Methods for isolation and contamination from liquids, solids, and surfaces
  • Upon completion of this course, the student will be able to successfully isolate, mount, and handle particles sized 1-100 µm from liquids, solids, and surfaces for future analysis by PLM, IR, Raman, SEM and TEM
  • Given a stereomicroscope and microtools, the student will be able to operate and use them effectively in sample preparation
  • Provided with techniques helpful to the operation and use of microscopical instrumentation and microtools, the student will perform precise isolation, mounting, and handling of micro particles
  • Instructed in microtool fabrication, the student will learn how to produce tools correctly to aid in the isolation, mounting, and handling of micro particles
  • Equipped with instruments, microtools, and techniques, the student will successfully isolate particles from liquids, semi-soft solids, and surfaces
  • This course is directed towards students who would like to learn the precise methods of particle isolation, mounting, and manipulation
  • Professionals that must prepare, isolate, mount, or manipulate particles for a wide range of analysis techniques will benefit from attending this class

All instrumentation, materials, and supplies necessary for successful completion of this course will be provided onsite by Hooke College of Applied Sciences.

  • Detailed course manual
  • Olympus SZX10 stereomicroscope for each student
  • Olympus BX51 polarized light microscope
  • Undergraduate science background and analytical chemistry
  • Students should be familiar with polarized light microscopy and stereomicroscopy, and micrometry as applied to microscopic specimens

Students are expected to successfully complete a variety of tasks in the form of hands-on exercises, laboratory exercises, identifications of unknowns, and quizzes. Students are notified at the end of the course whether or not they have successfully completed the requirements of the course based on:

  • 100% Attendance
  • Class participation
  • Completion of all course material
  • Completed and signed student evaluation form

Upon successfully meeting these requirements, a student is awarded a certificate of completion and CEU credits, if available. Those who have not successfully passed the course requirements do not receive a certificate or CEU credits.

Please see our General Policies page for information related to course registration and cancellation.

This course is part of the Industrial Microscopy Specialization program.

The program consists of attending four standard Hooke College of Applied Sciences courses with hands-on instruction and a 40 hour practicum study characterizing unknown samples.

  1. Polarized Light Microscopy
  2. Introduction to Scanning Electron Microscopy
  3. Sample Preparation: Particle Isolation and Handling Techniques
  4. FTIR Training

After finishing the four courses, students complete a Capstone Project that requires students to characterize and identify samples by integrating all of the skills learned during the four courses taken for the program. Those students who successfully complete the four courses and associated practicums and a Capstone Project earn an Industrial Microscopy Specialization.

Upcoming Dates


May 5, 2020 - May 7, 2020
$1895 | 2 credits
T - TH | 8am - 5pm
Instructor: Heidi M. Talesky
REGISTER
September 29, 2020 - October 1, 2020
$1895 | 2 credits
T - TH | 8am - 5pm
Instructor: Heidi M. Talesky
REGISTER

Instructors Who Teach This Course


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