Students learn to take digital photomicrographs for transmitted light and fluorescence microscopy. Through lectures, demonstrations, and laboratory exercises on stained sections and fluorescent specimens students are trained to record microscopical observations and ensure the capture of fine details to their microscope's limits of resolution. Course attendees learn from hands-on experience using the microscope, cameras, and computers.
See below for additional course information.
• Microscope: Effectively use an Olympus microscope equipped for transmitted light and epifluorescent illumination. The function and adjustment of the microscope controls will be mastered through lectures, demonstrations, and repeated hands-on practice.
• Cameras: The types of digital cameras with their technical descriptions will be discussed in lecture so the students can determine the best camera for their imaging needs. Demonstrations and laboratory exercises will be performed on the microscopes provided to the attendees by Hooke College.
• Consumer Interchangeable Lens Cameras: The advantages and disadvantages of using high pixel count, interchangeable lens cameras will be described and demonstrated. The role of HD, 2K, and 4K video as a microscope procedure will be discussed.
• Integrating Microscope and Camera: The connection between microscope and camera will be discussed. Students will work with their camera and microscopes to become familiar with the procedures for acquiring the best images. A discussion of camera and microscope resolution with be discussed with an emphasis on the importance matching the microscope's magnification to the physical size of the pixel.
• Pre and Post Processing of the Image: Procedures for setting up the microscope for digital imaging and for correcting or enhancing the image along with how to obtain a color image with a monochrome camera.
• Specimen Preparation: Fixation and mounting the slides for bright field and fluorescence, the role of stains in these two imaging dualities, plus the role of the mounting medium and coverglass.
• Image Enhancement: Guidelines for what is permissible in scientific publications as well as how they are covered.
College degree or familiarity with laboratory-grade microscope
Students are expected to successfully complete a variety of tasks in the form of hands-on exercises, laboratory exercises, identifications of unknowns, and quizzes. Students are notified at the end of the course whether or not they have successfully completed the requirements of the course based on:
Upon successfully meeting these requirements, a student is awarded a certificate of completion and CEU credits, if available. Those who have not successfully passed the course requirements do not receive a certificate or CEU credits.