Microtomy is a common method for preparing thin sections of the sample for TEM analysis. A diamond knife slices out a block of the material. This can be done under ambient conditions if the material is sufficiently hard, or under liquid nitrogen conditions if, for instance, a polymer needs to be frozen. Packaging materials, labels, multilayers and, in general, any plastics or rubber compounds can be cut and will often require staining with a heavy metal to provide contrast in the TEM. Some metals and oxides can also be microtomed, but most ceramics and minerals are too hard, have a tendency to chip the knife or are too brittle themselves and, therefore, break apart during the “controlled fracture” of the advancing knife. These materials require ion milling.
Essentials of Polarized Light Microscopy and Ancillary Techniques by John Gustav Delly has been published.
Call Us: 630.887.7100
Receive news, promotion, and event information from The McCrone Group.