Linkam Correlative Microscopy Stage
The CMS196V3 was designed specifically to solve the problem of how to get vitrified EM grids from the fluorescent microscope into the cryo TEM without devitrification and contamination through condensation.
- Read the article Imaging life in the freezer: Cryo-fluorescence microscopy in the January/February 2017 issue of Microscopy & Analysis, page 13.
- Read the study “Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins“.
- Read the study “Multiparametric analysis of the effectiveness of cisplatin on cutaneous squamous carcinoma cells using two different types of adjuvants“.
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The CMS196V³ is a cryo-CLEM instrument to enable the full workflow of CLEM. Apart from maintaining the sample vitrified by means of liquid nitrogen cooling it provides proven capabilities to safely handle and transfer cryo samples and image them with optical microscopy while keeping them free of contamination at all times. The integrated motorized, encoded stage of the latest model, together with the new LINK software enables high resolution maps of the grid to be produced quickly and easily. This provides a straight forward method of coordinate mapping required to locate the same sample in the fluorescence microscope as well as in the EM.
Developed in collaboration with Prof. Bram Koster’s team at Leiden University Medical Centre (LUMC) in the Netherlands, the CMS196 is the result of 3 years of R&D.
“The correlative stage was developed out of the necessity for a tool that uses fluorescence microscopy to help localize structures of interest in samples for high resolution cryo electron microscopy imaging. Over the years the system has evolved into an effective and efficient instrument that enables the imaging of cryo electron microscope images after light microscopy characterization routinely.” Prof. A.J. Koster – LUMC
Although the CMS196V3 was designed specifically to solve the problem of how to get vitrified EM grids from the fluorescent microscope into the cryo TEM without devitrification and contamination through condensation, the stage also had to be optimized optically to enable the use of high NA lenses.
Up to 3 grids are loaded into a specially designed cassette which is transported from your plunge freezer in a small sealed container. The container is loaded into a precooled dry sample loading chamber fitted on your upright fluorescent microscope. The cassette is then easily loaded onto the viewing bridge using special manipulation tools.
With more than 100 systems now in the field around the world, the CMS196 is the proven market leader in Cryo-CLEM.
Features/Highlights of the CMS196V³
- Self-contained cryo sample observation system keeping the sample vitrified at constant -196°C and avoiding contamination
- Automatic top-up liquid nitrogen top up of the chamber
- Short startup time of only 10 minutes, no mechanical connection between sample mount and Dewar, very good stability, low drift in nm range, good long-term stability
- Integrated motorized XY stage with optical encoders for high precision movement and position readout of better than 1µm
- Fully automated XY mapping
- Mounting option for most research grade, upright microscopes from all major manufacturers
- Self-aligning magnetic sample cassette system for up to three sample grids
- Support for EM-grids, Auto-Grids (FEI), Membrane carriers (Leica HPM100), Bessy grids, planchettes, CryoCapsule from CryoCapCell and custom sample holders—no need to handle bare grids
- Sample cassette holder for safe and contamination-free sample loading, storage and transfer
- Connects to plunge-freezing and High-Pressure Freezing sample preparation protocols
- Integrated sensors for sample temperature monitoring
- Integrated condenser optics for transmitted light brightfield and phase contrast
- Fluorescence observation with NA of up to 0.9
- Full day of independent operation with optional 3L autofill LN2 Dewar
- LCD display with button control, standalone joystick operation and full software control via USB interface
- Automated heating “bake-out” cycle to drive out moisture
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