Linkam Correlative Microscopy Stage
SKU:L-CMS196
The CMS196V4 was designed specifically to solve the problem of how to get vitrified EM grids from the fluorescent microscope into the cryo TEM without devitrification and contamination through condensation.
- Read the article Imaging life in the freezer: Cryo-fluorescence microscopy in the January/February 2017 issue of Microscopy & Analysis, page 13.
- Read the study “Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins“.
- Read the study “Multiparametric analysis of the effectiveness of cisplatin on cutaneous squamous carcinoma cells using two different types of adjuvants“.
The CMS196V4 is a cryo-correlative microscopy system enabling cryo-fluorescence, the full workflow of Correlative Light and Electron Microscopy (CLEM) and Cryo-super resolution.
While electron microscopy (EM) provides structural information at very high resolution, it can give only restricted insight into biological and chemical processes due to limitations in staining and sample preparation processes. Fluorescence microscopy on the other hand is a very sensitive method to detect biological, chemical and genetic processes and events inside living cells. Cryo-CLEM brings together the dual advantages of low temperature fluorescence and cryo-EM by imaging the same sample location with both techniques, while also superimposing the complementing information.
The CMS196V4 maintains the vitrified state of the sample by liquid nitrogen cooling and provides proven capabilities to safely handle and transfer your cryo-samples. These can then be imaged with optical microscopy whilst ensuring they remain free of contamination. The integrated, encoded and motorised XY stage enables the co-ordinate mapping required to find the same sample feature again on complementing modalities, for example TEM, FIB-SEM or X-ray systems.
SELF-CONTAINED AUTOMATED LN2 TOP-UP
The gentle liquid nitrogen top-up ensures samples remain vitrified whilst also protecting the sample from contamination with a supply of clean nitrogen vapor and a liquid cold-trap.
INTEGRATED ENCODED MOTORISED XY STAGE
The encoded, motorized stage provides a highly stable platform allowing high precision automated mapping of the complete EM grid with 1 μm resolution.
CRYO-SAMPLE INTERFACE AND COMPATIBILITY
Sample cryo-cassettes ensure safe sample loading, transfer and storage. One cassette can hold up to 3 EM-grids. Dedicated Cassettes (see table on back cover) support standard EM grids, Clipped Grids, Planchettes, Bessy, Si3N4 frames, CryoCapCell and JEOL CRYOARM™
INTERCHANGEABLE CRYO-BRIDGE
The CMS196V4 has an interchangeable cryo-bridge which enables the user to configure the stage for custom requirements.
CORDLESS HEATED MAGNETIC SAMPLE CHAMBER LID
The heated frost-free lid allows convenient viewing of the sample area. This is powered by self-aligning magnetic contacts without the need to plug in the lid.
INTEGRATED LED CONDENSER
The integrated LED condenser allows brightfield transmitted light, which is ideal for initial viewing of the grid and locating the sample.
JOYSTICK TOUCH SCREEN PANEL
The CMS196V4 can be configured and operated from the connected touch panel. The joystick wheels allow intuitive sample navigation and swift sample review without a PC. The stage can also be controlled via USB and is supported by multiple software packages.
AUTOFILL LN2 DEWAR WITH SMART FEATURES
Optional liquid nitrogen 3 litre capacity Dewar extends the unattended run time of the system for to up to 6 hours. In addition, gentle trickle-fill modes allow increased stability for longer acquisition runs.
MICROSCOPE COMPATIBILITY AND MOUNTS
The CMS196V4 is compatible with a wide range of research grade upright microscopes and high NA objectives. A quick-release mount further simplifies sample loading for some microscopes.
OPTIONAL REDUCED SAMPLE DRIFT, LENS HEATER
Superior drift performance delivered by the continuous filling of LN2 to the sample chamber, temperature-controlled stage body and an objective lens heater (diameter range 27-30 mm).
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