Tungsten needles have much finer tips. Needle 1 has a short blunt tip and is very sturdy; it is used mainly for scribing marks on metal surfaces. Needle 2 is not as sturdy as needle 1; it has a finer tip, used mainly for cutting particles from collodion. Needle 3 is very fine; it is used most frequently for single particle manipulation. Because the tip is rather fragile, it bends or breaks frequently. Many of these needles should be kept on hand since a few may be necessary to complete manipulation of a single particle. Not only are tungsten needles used for particle manipulation, but they can also be used when working with embryos and for glass blowing.
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Essentials of Polarized Light Microscopy and Ancillary Techniques by John Gustav Delly has been published.
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